•Muscle mass :

– Skeletal Muscle mass is made up of large number of individual muscle cells or myocytes.
-Myocytes are long and slender in appearance and are commonly called muscle fibers.
-Muscle fibers are arranged parallel to one another with some connective tissue in between and they are multinucleated.
– Fascia separates muscle mass from its neighboring tissues.
– Epimysium is a connective tissue sheath which covers the muscle beneath the fascia.
-In the muscle , the muscle fibers are arranged as bundles or fasciculi.
– These fasciculi are covered by perimysium .
– And endomysium is the layer which covers each muscle fibers.

Muscle fiber :

-These are cylindrical in shape and are 3cm in length.
– Muscle fibers are attached to tendon  which in turn attaches to the bones.
– Plasma membrane encloses each muscle fiber that lies beneath the endomysium and also called as sarcolemma.
-Sarcoplasm is the cytoplasm of the muscle.
* Structures present within the sarcoplasm are ;
(1) nuclei
(2) myofibrils
(3) mitochondria
(4)sarcoplasmic reticulum
(5) Golgi apparatus
(6) ribosomes
(7) glycogen droplets
(8) occasional lipid droplets .


– Nuclei are situated just beneath the sarcolemma and are oval or elongated .
– Each muscle fiber has one or more number of nuclei and in long muscle fibers many nuclei are present.

• Myofibril

√ Definition :

-These are the fine parallel filaments seen in sarcoplasm of the muscle cell.

√ Morphology :

– In muscle fiber cross section, the myofibrils are appears like a distinct dots within sarcoplasm.
– And these myofibrils are run along the entire length of muscle.
– Cohnheim’s areas are the fields where some of the myofibrils are arranged in groups .

√ Microscopic structure of a Myofibril :

– All the myofibrils are consists of a number of two alternative bands and are alled as the sections , segments or disks.
– Bands are formed by the muscle proteins.
– The two bands are;
(1) light band / ‘I’ band
(2) Dark band / ‘A’ band.

1) Light band 

– Light band  is isotropic to polarized light  hence it is called as isotropic or I band.
– When this polarized light passes through the muscle fiber at ‘I’ band area , the light rays are refracted at the same angle.

2) Dark band

-Dark band is anisotropic to polarized light  , hence it is called as anisotropic or A band .
-When this light passes through muscle fiber at this area , the light rays are refracted at different directions .
– It is also called as ‘ Q’ disk.

• Sarcomere 

√ Definition :

– It is the structural and functional unit of the skeletal muscle.
-Also known as basic contractile unit of the muscle.

Extent :

– It extends between two ‘Z’ lines of myofibrils.
-The average length when muscle is in relaxed state  is  2- 3 micro.

√ Components :

(1) one half of light ‘I’ band
(2) one dark ‘A’ band
(3) one half of light ‘I’ band.
–  ‘H’ zone is the light area present in the middle of ‘A’ band
– ‘M’ line is the middle part of myosin filament lies in the middle of ‘H’ zone and this line is formed by the myosin binding protein.

•Electron microscopic study of Sarcomere:

– Myofilaments are the thread like structure present in the Sarcomere.
-They are of two types
(1) Actin filaments : Thin filaments
(2) Myosin filaments : Thick filaments.
• Contractile proteins of muscle:
1) Myosin molecule
2) Actin molecule

1) Myosin molecule :

– About 200 myosin molecules are present in each myosin filament.
– Two portions of myosin molecule;
 ✓ Tail portion 
✓ Head portion 

Tail portion : 

– Formed by heavy chains and both the heavy chains are twist around each other as a double helix.

Head portion :

– Formed by the heavy chains and light chains.
– Both the heavy chains turn away in opposite directions at one end of the double helix and form the globular head portion.
– Each part of the head portion of myosin molecule consists of two light chains.
 ✓ essential light chain : regulate structural stability of myosin head
 ✓ regulatory light chain : regulates kinetics of myosin head.
– There are two attachment sites in each myosin head .
– One is for actin filament attachment
Another one is for ATP molecule attachment.

2) Actin molecule :

– Each actin molecule is called as F-actin and they are the major constituents of the thin actin filaments.
– It is a polymer of G-actin .

3) Tropomyosin:

– In the double helix strand of actin filament consists of about 40 to 60 Tropomyosin molecules.
– And in relaxed condition of the muscle, these molecules cover all active sites of the F-actin molecules.

4) Troponin :

– It includes three substitues,
(1) Troponin I  : attached to F- actin
(2) Troponin T : attached to Tropomyosin
(3) Troponin C : attached to calcium ion.
* Other proteins of the muscle ;
(1) Actinin – attaches actin filaments to ‘Z’ line
(2) Desmin – attaches ‘Z’ line with sarcolemma.
(3)Nebulin – runs in parallel to actin filaments
(4) Titon – connects ‘M’ line and ‘Z’ line.

• Sarcotubular system :

– It is a system of membranous structure in form of the vesicles and the tubules in sarcoplasm of muscle fiber .
– Myofibrils are surrounded by the Sarcotubular system .
* This system is formed by the 2 types of structures ;
(1) T- tubules 
(2) L- tubules.

1) T- tubules :

✓ Transverse tubules are formed by the invagination of sarcolemma and they are narrow tubules.
✓These tubules penetrate from one side of the muscle fiber to another side.
✓ The T- tubules open to the exterior of the muscle cell  and therefore , the ECF runs through their lumen .


2) L- tubules :

✓These are the closed tubules which runs in the long axis of the muscle fibers and form sarcoplasmic retinaculum.
✓L- tubules do not open to exterior of the muscle cell.
✓ At regular intervals , the L- tubules dialates to form terminal cisternae.
✓ Terminal cisternae is in close contact with T- tubules.
✓ On either side , T- tubules along with the cisternae is called the triad of skeletal muscle.
✓ In human skeletal muscle , these triad are situated at the junction between Anisotropic band and isotropic band.
✓ Calcium ion are stored in L- tubules and are more in cisternae.

• Functions of Sarcotubular system:

(1) T- tubules :

– This tubules are responsible for the rapid transmission of the nerve impulse to form the action potential from sarcolemma to the myofibrils.

(2) L- tubules :

– This tubule stores large number of calcium ions.
– When the action potential reaches the cisternae of the L- tubules , calcium ions are releases into the sarcoplasm.

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